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Lab 8 Central Dogma Lab (DNA RNA Protein)

Lab 8 Central Dogma Lab (DNA RNA Protein)
Instructions
⦁ All work needs to be your own. This is the most important instruction. Evidence this work is not your own can lead to a zero and a report to the Dean of Students for academic dishonesty. It is not worth it. Your answers do not have to be perfect to earn an excellent grade. If you are having trouble, tell me before the deadline. It is my job to help you work through this material.
⦁ Put your answers in bold or highlight.
⦁ To help you find the questions you need to answer, those questions begin with red highlight.
⦁ Information from this lab will be tested on Exam 3 Life’s Information.

Part 1 of 4: DNA Extraction
⦁ Go to this website http://learn.genetics.utah.edu/ or click google “learn genetics Utah.”
⦁ Click on the tab towards the bottom right of the home page for “Virtual Labs” and then click on “Gel Electrophoresis.”
⦁ If you don’t have flash player for this animated virtual lab, you can watch this video of the lab instead: https://youtu.be/m_3YUbriCxM

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Answer the following questions as you work your way through the lab.
⦁ What are THREE reasons one would need to isolate human DNA?

⦁ What does DNA need to be isolated/purified from?

⦁ How much DNA is in every nucleus of every human cell?

⦁ Where are the cells that are used as the DNA source in this lab?

⦁ How are the cells collected?

⦁ What does lysis mean?

⦁ What does the detergent in a lysis solution do?

⦁ What does the proteinase in a lysis solution do?

⦁ Why add salt solution?

⦁ What does the centrifuge separate?

⦁ What is in the liquid portion after separation?

⦁ What is in the clump at the bottom after separation?

⦁ Why add alcohol?

⦁ Why use the centrifuge again?

⦁ If you would like to try to extract DNA at home, here is the link https://learn.genetics.utah.edu/content/labs/extraction/howto/

Part 2 of 4: DNA Replication, Predicting Sequence of Complementary DNA
Write the complementary sequence in box #2, 4, 6
Template DNA sequence
3’-C A T G G C A T A C C A A A T A C G-5’
Complementary DNA EXAMPLE 5’-G T A C C G T A T G G T T T A T G C -3’

1. Template DNA sequence 3’-T A C T G A A C A C A T G C G C C C-5’
2. Complementary DNA 5’-

3. Template DNA sequence 3’-A G T C C A C C G G T T T A T C T C-5’
4. Complementary DNA 5’-

5. Template DNA sequence 3’-T T C A G T C C A A A T T T T G C G-5’
6. Complementary DNA 5’-

Part 3 of 4: RNA Transcription, Predicting Sequence of Complementary RNA
Write the complementary RNA sequence in box #2, 4, 6 (no Ts)
Template DNA sequence
3’-C A T G G C A T A C C A A A T A C G-5’
Complementary RNA EXAMPLE 5’-G U A C C G U A U G G U U U A U G C-3’

1. Template DNA sequence 3’-T A C T G A A C A C A T G C G C C C-5’
2. Complementary RNA 5’-

3. Template DNA sequence 3’-A G T C C A C C G G T T T A T C T C-5’
4. Complementary RNA 5’-

5. Template DNA sequence 3’-T T C A G T C C A A A T T T T G C G-5’
6. Complementary RNA 5’-

Part 4 of 4: Protein Translation

Write the translated amino acid sequences in box #2, 4, 6 (no Ts).
Each three RNA letters = one codon
1. Template RNA sequence 5’- A U G -A U U -G C A -C G U -U A G -3’
2. Amino Acid Sequence EXAMPLE Met Ile Ala Arg stop

1. Template RNA sequence 5’- A U G U A C G U C A G U U A A -3’
2. Amino Acid Sequence

3. Template RNA sequence 5’- A U G A A A C C U G U U U A G -3’
4. Amino Acid Sequence

5. Template RNA sequence 5’- A U G G G C A A U C C C U G A -3’
6. Amino Acid Sequence

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